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Laboratory products
The laboratory of the GOOD CELLS medical company produces a wide range of biotechnological cellular and cell-free products for regenerative medicine.
● Safe stem cells ●
Only quality and safe biotechnological products from GOOD CELLS!
Any biotechnological product is screened for infectious lesions (HIV½, HBV, HCV, HSV½, CMV, EBV, Treponema pallidum, Mycoplasma homini, Mycoplasma genitalium) and for compliance with the criteria for determination (phenotype, proliferative activity, ability to form colonies, proliferation potential). The results of these are stored in the individual passport of the biotechnological product.
That is why we are 100% confident in our products' quality and take responsibility for the result.
Multipotent mesenchymal stromal/stem cells
Mesenchymal stem cells (MSCs) are postnatal, self-renewing, and multipotent stem cells giving rise to all the skeletal tissues.
The broad-range of MSCs therapeutic potential could be explained by their biological properties: proliferation potential, multipotency potential (ability to differentiate under the appropriate stimulus and replace the damaged cells), trophic function, homing/migration (ability to find damaged tissues or own native niches after systemic adoptive transfer), immunosuppression function (MSCs suppress the proliferation and activity of CD4+ T helper cells, CD8+ cytotoxic T cells, pro-inflammatory macrophages, neutrophils, natural killer cells, and B cells. At the same time, MSCs stimulate regulatory T and B cells, anti-inflammation macrophages, and immature dendritic cells).
The biotechnology laboratory of GOOD CELLS allows to obtain different MSCs types.
1. Bone marrow multipotent mesenchymal stromal/stem cells (BM-MSCs) — a cell product derived from human red marrow. BM-MSCs rapidly proliferate, have potential for tri-lineage differentiation (adipocytes, chondrocytes, and osteocytes), also clonogenic potential.
2. Adipose-derived multipotent mesenchymal stromal/stem cells (ADSCs) — a cell product derived from lipoaspirate of human subcutaneous fat. ADSCs are a prospective candidate for regenerative medicine for several reasons: ease to harvest and isolate; high proliferation rate, stable doubling time in comparison to BM-MSCs, multilineage differentiation potential, immunotolerance, innate ability induces angiogenic traits, anti-inflammatory properties, either autologous and allogeneic use.
3. Umbilical cord multipotent mesenchymal stromal/stem cells (UC-MSCs) — a cell product derived from the connective tissue of the umbilical cord. The umbilical cord contains two arteries and one vein, protected by a proteoglycan rich connective tissue called Wharton's jelly. Wharton's jelly is one of the mesenchymal stem cell sources. However, in comparison to bone marrow and adipose tissue that are classical MSCs providers, the number of MSCs isolated from the umbilical cord can be several times higher. Noninvasive collection procedure, high proliferation potential, a steady doubling time (DT), high frequency of clonogenic cells, broad differentiation potential, low immunogenicity are attractive advantages of UC-MSCs. These make the umbilical cord the predominant source of MSCs.
4. Placental multipotent mesenchymal stromal/stem cells (P-MSCs) — a cell product derived from the placenta. Placenta is a unique organ composed of maternal and fetus cells. Along with this, placenta is a high-yielding reservoir of mesenchymal stem cells. There are several advantages of P-MSCs in comparison to other adult MSCs:
● more homogeneous and "primitive" cells population;● high proliferation rate;● low risk of ex vivo senescence;● enhanced properties for homing to damaged tissue;● immunotolerance.
5. Amniotic membrane multipotent mesenchymal stromal/stem cells (AMSCs) — a cell product derived from the outer mesodermal layer of the amniotic membrane. The amniotic membrane, or amnion, is an extraembryonic highly resilient, transparent membrane that encompasses the fetus during development. This structure is generated very early in development and consists of three discrete layers: an inner epithelial layer, an interposing, acellular basement membrane, and an outer layer of mesodermal cells. Therefore, the amnion is a source of at least two stem cell types: amniotic membrane mesenchymal stem cells (AMSCs) and amniotic membrane epithelial cells (AECs). AMSCs arise from the outer mesodermal layer. AMSCs shared similar properties with placental MSCs, but also are characterized by the predisposition for neural differentiation.
6. Endometrium-derived multipotent mesenchymal stromal/stem cells (En-MSCs) — a cell product derived from a healthy endometrial area. En-MSCs are a new class of adult stem cells with high proliferation activity, multilineage differentiation potential, low immunogenicity, and endometrium tropism. En-MSCs are isolated from endometrium tissue sample 0.1-20 mm in size obtained by minimally invasive pipelle biopsy during the routine uterus diagnostic procedure. Besides classical MSCs characteristics, en-MSCs express receptors to progesterone (PGR) and estrogen (ESR1 and ESR2) as well as exclusive en-MSCs markers SUSD2, also have a unique cytokine and matrix metalloproteinase (MMP) profile.Neural crest-derived multipotent stem cells
Numerous studies demonstrated that neural crest stem cells (NCSCs) have been identified not only during embryonic development but also in adulthood. In humans, stem cells with neural crest properties are stored in the hair follicle, skin dermis, adipose tissue, bone marrow, palate, nasal mucosa, dental pulp, etc.
Human NCSCs isolated from different tissues are characterized by the following biological properties:
● high proliferation rate;● high migration potential;● capability to self-renew;● ability to form neuro-like spheroids in specific serum-free growth environment or on cultural plates with a non-adhesive surface;● the display of the key neural crest cells markers — SOX10, CD271 (P75, LNGFR), NESTIN, SOX2, SNAIL1, SNAIL2, TWIST1, TFAP2A;● the expression of the MSCs markers — CD73, CD90, and CD105;● the expression of the pluripotency and stemness markers — OCT3/4, KLF4, c-MYC, and NANOG;● NCSCs are negative for CD34, CD45, HLA-DR, CD56, and CD117 expression;● rich spectrum of secretome — IL-2Ra, IL-3, IL-5, IL-8, IL-16, MCP-1, SDF1a, GM-CSF, M-CSF, VEGF, HGF, bFGF, NGF etc.;● the ability for multilineage differentiation in vitro — adipocytes, chondrocytes, osteocytes, myocytes, melanocytes, neuronal cell lineages, Schwann cells;● cell plasticity allows NCSCs to be well integrated structurally and functionally after transplantation in the recipient’s tissues and organs;● non-tumorigenic in vivo.
All the biological properties of NCSCs mentioned above, together with several accessible sources in the adult body, make these cells attractive candidates for regenerative medicine, especially in neurology.
Biotechnologists of the laboratory successfully isolate, grow, and determine the NCSCs from:
● hair follicle neural crest stem cells (HF NCSCs) — a cell product obtained from human hair follicles. Hair follicles grow during all life, and the hair cycle is a well-controlled process involving stem cell proliferation and quiescence. Hair bulge is a well-characterized niche for adult stem cells, including epithelial stem cells, melanocyte stem cells, and neural crest-like stem cells. The hair follicle is isolated from the skin specimens of the scalp zone under local anesthesia by a dermal punch knife. It is a safe and minimally invasive procedure. HF NCSCs are self-renewed, multipotent stem cells that express specific markers of specialized cells and display appropriate functions in ex vivo conditions. Besides HF NCSCs, our biotechnologists can isolate dermal fibroblasts and adipose-derived MSCs from the same skin specimen. These cell types could be grown to the needed quantity and stored until usage.
● skin-derived neural crest stem cells (SD NCSCs) — a cell product obtained from skin specimens of the scalp zone under local anesthesia by minimally invasive punch biopsy. SD NCSCs rapidly proliferate, are prone to multilineage differentiation (adipocytes, chondrocytes, osteocytes, neurons, and Schwann cells), have clonogenic potential.
● dental pulp neural crest stem cells (DP NCSCs) — a cell product obtained from the dental pulp of deciduous human teeth. Dental pulp of human teeth is a rich source of stem cells with the properties of NCSCs. The isolation of stem cells from dental pulp is a common noninvasive surgical practice that can be performed in the adult during life: orthodontic extraction, wisdom tooth extraction, exfoliated deciduous. It should be noted that isolation of DP NCSCs is one of the possibilities toward personalized medicine via own cell banking. DP NCSCs are characterized by huge therapeutic potential due to high proliferation rate, ability to form a neurosphere-like structure in appropriate conditions, expression of numerous markers that are specific for neural crest cells and nervous system cells (NESTIN, MUSASHI-1, p75, CD56, SNAIL-1,-2, SLUG, SOX-9, SOX-2, neurofilament, glial fibrillary acidic protein, etc.), angiogenic potential (DP NCSSCs secrete PDGF, bFGF, VEGF, ANG, ANGPT1, CSF, EDN1, IL-8, IGFB3, MCP-1), multilineage differentiation potential (adipogenic, chondrogenic, osteogenic, myogenic, dentonogenic, neurogenic), possessing immunomodulatory properties by expressing IL-6, IL-8, TGF-β, indoleamine 2,3-dioxygenase.
● palate-derived neural crest stem cells (pNCSCs) — a cell product isolated from oral mucosa samples of the hard palate (paracrestal location) during elective dentoalveolar surgery.Amniotic membrane epithelial cells
Amniotic membrane epithelial cells (AECs) — a cell product derived from the inner layer of the amniotic membrane. In vitro, the AECs show epithelial morphologies and grow into a tightly packed, cobblestone monolayer in culture. The ease of AEC isolation, their ability to differentiate into all three germ layers, their low immunogenicity, anti-inflammatory properties, and their noncontroversial nature make them an up-and-coming tool for application in regenerative medicine.
Cord blood mononuclear cells
Cord blood mononuclear cells is a cell product that represents mononuclear cells collected from cord blood — a mixture of nuclear cells including leukocytes and different types of stem cells (hematopoietic stem cells, MSCs, endothelial progenitors).
Periosteal progenitor cells
Periosteum derived progenitor cells (PDPCs) — a cell product derived from the periosteum of fibula fragment obtained under the surgical procedure. The periosteum is a specialized and highly vascularized connective tissue that envelopes bone surfaces. It consists of an external fibrous layer containing elastic fibers and microvessels, and an inner cambium layer where PDPCs reside that act as major players in bone development and fracture healing. PDPCs in vitro demonstrate rapid proliferation, ability to differentiation, express CD73, CD90, CD105, CD146, CD166, NESTIN, negative for expression CD45, HLA-DR and CD34. Due to the intrinsic potential to form bone, PDPCs is the better choice for bone regeneration.
Endothelial progenitor cells
Endothelial progenitor cells (EPCs) — a cell product obtained from peripheral blood mononuclear cells. EPCs are unipotential progenitor cells involved in neovascularization and endothelium turnover by giving rise to endothelial cells. EPCs are capable of proliferation, tube formation in vitro, and in vivo. They express endothelium and stem cell-specific markers — CD34, CD31, CD144, VEGFR2, CD73, CD105.
Dermal fibroblasts
Dermal fibroblasts is a cell product derived from the dermis of skin samples obtained by minimally invasive punch biopsy. Fibroblasts are the main cell type of connective tissues that produce extracellular matrix (collagen, glycosaminoglycans, and elastin), maintaining organ and tissue integrity. Fibroblasts are involved in processes of wound healing, skin aging, scar formation, fibrosis development. In vitro fibroblasts are characterized by spindle-shaped morphology, express vimentin and CD90 markers, could differentiate in three lineages of mesenchymal direction (adipocytes, chondrocytes, and osteocytes), and even possess immunosuppressive properties.
Blood-derived products
1. Platelet-rich plasma (PRP) is a derived plasma fraction of blood that contains high concentrations of platelets (in 3 to 5 times greater than physiologic concentration) and growth factors with mitogenic and chemotactic proprieties.
PRP's main mechanism of action is through platelets, which besides their well-known function in homeostasis, release factors that promote angiogenesis and tissue repair. Secretory granules of the platelets contain growth factors, coagulation factors, cytokines, adhesion molecules, and integrins.
The main growth factors included in alfa granules are: ● platelet-derived growth factor (PDGF);● platelet-derived endothelial growth factor (PDEGF);● vascular endothelial growth factor (VEGF);● epidermal growth factor (EGF); ● insulin like growth factor (IGF);● platelet-derived angiogenesis factor (PDAF); ● transforming growth factor (TGF);● keratinocyte growth factor (KGF);● fibroblast growth factor (FGF);● connective tissue growth factor (CTGF);● tumor necrosis factor alpha (TNF-α);● interleukin-1β (IL-1β); ● interleukin 8 (IL-8).
All these growth factors stimulate healing by attracting cells in the newly formed matrix and triggering the cells' division. Another process involved in wound healing may be the role of PRP in suppressing the cytokine release that limits inflammation, and also in interaction with macrophages to promote tissue healing, regeneration, and epithelialization.
PRP is widely used in practical medicine. Musculoskeletal diseases (tendonitis, arthritis, ligament sprains, athletic injuries), plastic surgery (chronic, diabetic, and pressure ulcers), various gynecological disorders are subjects for PRP applications.
2. Platelet-poor plasma (PPP) — a derived plasma fraction of the blood with the concentrations of platelets below baseline levels in whole blood, but with a high volume of fibrinogen.
3. Blood mononuclear cells, autologous, a mixture of blood-derived nuclear cells obtained from patients' venous peripheral blood by centrifugation under gradient solution. This mixture contains leukocytes (95%) and different types of stem/progenitor cells.Adipose tissue-derived products
The GOOD CELLS company offers products received from the adipose tissue.
1. Stromal vascular fraction (SVF) is a heterogeneous population of cells derived from the patient's own adipose tissue that includes progenitor cells and stem cells with powerful regenerative potential, anti-inflammatory, and immunoregulatory action.
2. Nano fat — an emulsion from the patient's own adipose tissue obtained by machining adipose tissue and subsequent filtration. The emulsion consists of a stromal-vascular fraction (SVF) and some non-cellular elements of adipose tissue but devoid of viable adipocytes. SVF is the main active component of nano fat, which promotes tissue regeneration, induces collagen synthesis, endothelial cell proliferation, etc.
3. Micro fat, compared to nano fat, contains whole and viable adipocytes and their surrounding cellular environment. When administered, these adipocytes act as traditional fat grafts, embedded in the injection site.
Nano fat and micro fat are used to fill bags under the eyes, fine lines in the mouth, chest, buttocks, and general skin rejuvenation, where thin lines and skin exhaustion are found. Nano fat and micro fat are an alternative to traditional treatment methods with Botox, derma-filler, or other implants. Nano fat injections against wrinkles are safe and effective, don't cause an allergic reaction, and are performed on an outpatient basis. In addition, the results of this method last much longer than conventional methods because they stimulate facial skin regeneration at the molecular level. Long-term effects are also because the body's own fat is biocompatible and very well absorbed.
4. Mesenchymal stem cells from adipose tissue.Exosome-containing biotechnological product GoodEXOCells
The biotechnological product is used to reduce the severity of hair loss and stimulate scalp regeneration and hair growth. It can be used alone or in specialized dermatological and cosmetic clinics.
This product's safety and efficacy work well for the treatment of various pathological conditions, including alopecia, chronic and acute ischemia of the lower extremities, acute and chronic wounds, myocardial infarction, liver failure, stroke, spinal cord injury, bone defects.
The advantages of the exosome-containing product are that it is a ready-to-use, stable product enriched with growth factors and extracellular vesicles of allogeneic multipotent mesenchymal stromal/umbilical cord stem cells. One vial of the finished product is designed for one course of treatment. The use does not require special skills and medical supervision.
Find more information about the product on the GoodEXOCells website.